The left arm of homology (LA) contains about 280 nucleotides (e.g.
SEQ ID NO:13), for example, of identical sequence upstream
of the albumin intron 1 cleavage site, and the right arm
of homology (RA) contains, for example, about 100 nucleotides
(e.g. SEQ ID NO:16) of identical sequence downstream of
the cleavage site. The left arm of homology (LA) contains about 280
nucleotides (e.g. SEQ ID NO:13) of identical sequence upstream of the albumin intron 1 cleavage site, and the right arm of homology (RA) contains about 100 nucleotides (e.g.
SEQ ID NO:16) of identical sequence downstream of the cleavage site of the ZFNs useful in the methods and compositions disclosed
herein. In some embodiments, the ZFN is albumin-specific, for example, and the
halves (left and right components) of the albumin-specific ZFNs are
carried by separate AAV vectors. The method and compositions disclosed herein may be administered separately, or, preferably a composition comprising
all components (e.g. paired ZFNs on the same or different vectors and IDS donor), for example
a composition which comprises SB-47171 AAV (e.g. Table 1), SB-47898 AAV (e.g.
Table 2) and SB-IDS AAV (e.g. Table 3). In some embodiments, the composition comprises SB-71557 AAV (e.g.
Table 4, SEQ ID NO:30); SB-71728 (e.g. Table 5, SEQ
ID NO:31); and SB-IDS AAV (e.g. Table 3, SEQ ID NO:17).
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By way of introduction, I am Mark Schaefer, and I represent
Nutritional Products International.
We serve both international and domestic manufacturers who are seeking to gain more distribution within the
United States. Your brand recently caught my attention, so I am
contacting you today to discuss the possibility of expanding your national distribution reach.
We provide expertise in all areas of distribution, and our offerings include the following:
• Turnkey/One-stop solution
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and retailers
• Our executive team held executive positions with Walmart and Amazon
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lines
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Our history allows us to have intimate and unique relationships with key buyers across the United States,
thus giving your brand a fast track to market in a professional manner.
Please contact me directly so that we can discuss your brand further.
Kind Regards,
Mark Schaefer
marks@nutricompany.com
VP of Business Development
Nutritional Products International
101 Plaza Real S, Ste #224
Boca Raton, FL 33432
Office: 561-544-0719
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